dnase i (Zymo Research)

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Zymo Research dnase i
Dnase I, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+digestion+buffer/DNase+I+Set+w%2F+DNA+Digestion+Buffer/pm42049898-284-15-17
Average 95 stars, based on 490 article reviews

dnase i - by Bioz Stars, 2026-09

95/100 stars

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Incubation:

Article Title: Transcriptome of Taenia solium during in vitro cyst activation and initial growth into the tapeworm stage
Article Snippet: The protocol included DNAse I treatment (Zymo Research, E1009-A (250 U)). .. As recommended by the manufacturer, a mix of 5 μL of DNase I (1 U/μL) and 75 μL of DNA Digestion Buffer (Zymo Research, R1058) was added directly to the silica column matrix (Zymo-SpinTM IIICG Columns) containing processed samples, for a 15-min incubation at room temperature (20–30 °C). .. RNA yield was measured by spectrophotometry with NanoDrop® (Thermo Fisher Scientific, Waltham, MA, USA), and approximately 300 ng of RNA were loaded into a 1.5% agarose/1% bleach gel to test its integrity by electrophoresis (35 minutes at 100 V) .

Article Title: C/EBPβ-induced alternative splicing of RCAN1 generates a potent TCR-T target in mesenchymal glioblastoma
Article Snippet: The cell pellets were resuspended in 350 μL of Tri Reagent (Zymo Research, Cat. #R2050-1-200) and vortexed to mix the samples. .. A total of 350 μL of molecular grade ethanol (Decon Laboratories, Cat. #CAS# 64-17-5) was added to the TRIzol mixture and placed on a Zymo Spin IIICG Column (Zymo Research, Cat. #C1006-50-G) was placed in a collection tube (Zymo Research, Cat. #C1001-50) and spun at 16,000 × g for 30 s. Next, 400 μL of Zymo Wash Buffer (Zymo Research, Cat. #R1003-3-48) was added to the columns and centrifuged at 16,000 × g for 30 s. Next, 5 μL of DNase I (6 U/μL) (Zymo Research, Cat. #E1011-A) and 75 μL of DNA Digestion Buffer were mixed and placed onto the columns (Zymo Research, Cat. #E1010-1-16) and incubated at room temperature for 15 min. Next, 400 μL of Direct-Zol RNA Prewash (Zymo Research, Cat. #R2050-2-160) was added to the column and spun at 16,000 × g for 30 s. This step was then, 700 μL of RNA wash buffer was added to the column and centrifuged for 1 min at 16,000 × g . Finally, the RNA was eluted from the columns with 50 μL of DNase/RNase-free water (Zymo Research, Cat. #W1001-30) and spun at 16,000 × g for 30 s. After the RNA was isolated, 5 μL of RNA was used to ensure quality control, which was run on a 4200 Agilent Tape Station (Agilent, Cat. #G2991BA) using high-sensitivity RNA screen tapes (Agilent, Cat. #5067-5579). .. Bulk RNA was extracted with a Quick-DNA/RNA MiniPrep Kit (Zymo Research, Cat. #D7001) following the manufacturer’s protocol.

Article Title: Injectable aptamer-functionalized nucleic acid-collagen hydrogels as a bioactive platform for angiogenic applications.
Article Snippet: Nucleic acid-collagen complexes (NACCs) have gained prominence as multifunctional biomaterials fabricated by the self-assembly of type I collagen and single-stranded DNA (ssDNA).. This study reports injectable NACCs functionalized with an ssDNA aptamer that activates the vascular endothelial growth factor receptor 2 (VEGFR2), organizing into a hydrogel capable of promoting localized angiogenesis.. The protection of the embedded aptamer from degradation was confirmed under nuclease-rich conditions, and rheological analysis demonstrated the shear-thinning behavior of the hydrogel, supporting injectability through a 25G needle.

Article Title: Correlated protein-RNA associations reveal a requirement for HNRNPU in long-range Polycomb recruitment by the lncRNAs Airn , Kcnq1ot1 , and Xist
Article Snippet: Samples were vortexed and applied to Zymo-Spin IC Columns (Zymo #R1013) and spun for 30 s at top speed on a benchtop microcentrifuge. .. Next, 400 μL of RNA Wash Buffer (Zymo #R1013) was added, and the samples were spun at top speed for 30 s. For each sample, 5 μL DNase I and 35 μL of DNA Digestion Buffer (Zymo #R1013) was added directly to the column matrix and incubated at room temperature for 20 min. Next, 400 μL of RNA Prep Buffer (Zymo #R1013) was added, and the columns were spun at top speed for 30 s. Next, 700 μL RNA Wash Buffer (Zymo #R1013) was added, and the columns were spun at top speed for 30 s. Next, 400 μL RNA Wash Buffer was added, and the columns were spun at top speed for 30 s. The flow-through was discarded, and the columns were spun again for 2 min to remove all traces of the wash buffer. ..

Article Title: Xist Repeat A coordinates an assembly of SR proteins to recruit SPEN and induce gene silencing
Article Snippet: A 400-μL volume of RNA Wash Buffer (Zymo R1013) was added to each column and centrifuged (16,100 x g, 30 s, room temp). .. To each column, a 40-μL volume of a mixture containing 5 μL DNase I (1 U/μL) and 35 μL DNA Digestion Buffer (Zymo R1013) was added; samples were incubated at room temp for 20 min. A 400-μL volume of RNA Prep Buffer (Zymo R1013) was then added, and columns were centrifuged (16,100 x g, 30 s, room temp). ..

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Article Title: C/EBPβ-induced alternative splicing of RCAN1 generates a potent TCR-T target in mesenchymal glioblastoma.
Article Snippet: Bulk RNA extraction and library construction Bulk RNA was extracted with a Quick-DNA/RNA MiniPrep Kit (Zymo Research, Cat. #D7001) following the manufacturer’s protocol.

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